- Animal Resources Core
- Bioinformatics Core
- DNA Sequencing Core
- General Instrumentation Core
- Microscopy Core
- External Resources
Animal Resources Core
The Animal Resources Core provides extensive expertise and facilities for zebrafish husbandry and experimental use. The Core is equipped with Aquaneering aquatic systems in four dedicated husbandry rooms. Expertise and resources are available to perform:
- Custom platforms for antisense morpholino and cDNA microinjection
- Fully automated Zebralab workstation to conduct high content imaging and behavioral assays
- iWorx ECG system for cardiotoxicity studies
- Adaptable and scalable drug screening and assay development
Zebrafish resources are managed by Voot Yin, Ph.D.
The Core also provides access to diverse invertebrate and non-mammalian vertebrates, including local marine organisms as well as experimental models such as Xenopus laevis.
General animal resources are managed by Mark Hanscome.
The Bioinformatics Core collaborates with scientists to provide essential data management and analysis tools along with experimental design consulting using continually updated workflows. The Core also specializes in custom database and analysis software development.
A major initiative of the Core is the development of the Comparative Models of Regeneration Database (RegenDB) to provide a systems-level view of regenerative biology and stem cell self-renewal. This resource is part of the MDI Biological Laboratory’s Center of Biomedical Research Excellence (COBRE) that focuses on regeneration, tissue repair, and aging.
The Core also serves as a statewide resource for the Maine IDeA Network for Biomedical Research Excellence (ME-INBRE) program in comparative functional genomics by providing essential consulting and training in bioinformatics.
The Bioinformatics Core is directed by Benjamin L. King, Ph.D.
DNA Sequencing Core
The DNA Sequencing Core provides an array of molecular tools for MDI Biological Laboratory investigators. Two Applied Biosystems 3130xl DNA sequencers support traditional Sanger sequencing and fragment analysis. Multiple real-time PCR platforms are available for gene expression studies, including Agilent Mx3005p and Mx3000p instruments, a Roche LightCycler 480 II with interchangeable 96- and 384-well modules, and a Roche LightCycler 1536 with an associated Innovadyne Screenmaker 96+8 liquid handling workstation.
Two Agilent 2100 Bioanalyzers, a Nanodrop spectrophotometer, and an Invitrogen Qubit fluorometer provide quantification and quality assessment of nucleic acids and other samples. A Syngene G:Box Chemi gel documentation system captures DNA and protein gel images and chemiluminescent Western blots.
To make a sequencing request, see Instructions for Submitting Samples.
The DNA Sequencing Core is staffed by Christine Smith.
General Instrumentation Core
The General Instrumentation Core provides access to scientific instrumentation including high speed and ultra centrifuges, scintillation counters, cryostats, plate readers, spectrophotometers, and gel-imaging systems.
The General Instrumentation Core is managed by Aric Rogers, Ph.D.
The Microscopy Core provides numerous microscopes as well as trained staff to assist in their use. The microscopes include two laser scanning confocal microscopes, a Zeiss LSM 510 Meta and an Olympus Fluoview 1000. Both are capable of collecting transmitted light as well as epifluorescence data and can be used for time-lapse imaging, Fluorescence Resonance Energy Transfer (FRET), and Fluorescence Recovery After Photobleaching (FRAP) experiments.
In addition, three Zeiss Axiovert 200 inverted compound microscopes are available for high resolution phase contrast, differential interference contrast, Hoffmann modulation contrast, and widefield epifluorescence microscopy, as well as digitally enhanced real time and time lapse video microscopy and low light video microscopy.
Two stereomicroscopes, an Olympus FVX10, and a Zeiss V12 Discovery are available for transmitted light and epifluorescence imaging of C. elegans, zebrafish embryos and larvae, and other larger species and specimens.