Endogenous neural stem cell reprogramming for neuronal regeneration
In humans, olfactory or optic nerve injuries and associated neuronal degenerative diseases are often followed by permanent loss of smell or vision respectively. Unlike in humans, the zebrafish has the capacity to fully regenerate all neuronal subtypes and restore sensory functions after injury. Understanding the molecular mechanisms controlling retinal and olfactory neural stem cell function in zebrafish provides a starting point to design future strategies fully exploiting mammalian NSC potential for neuronal regeneration. The Madelaine Lab recently identified transcription factors involved in developmental and regenerative neurogenesis. This research project will study the capacity of these factors to activate/reprogram endogenous NSCs, allowing the formation of new neurons. They expect their discoveries to open new therapeutic strategies leading to the regeneration of neurons, limiting the negative impact of neurodegenerative diseases in humans.
Apelin signalling dependent muscle regeneration and rejuvenation
Progress in biomedicine has allowed people to live longer, but with increasing age, elderly people often suffer from age-related diseases. The degenerative loss of muscle leading to muscular atrophy is called sarcopenia. The onset of sarcopenia has recently been linked to a decline in muscle regenerative capacity because of defects in muscle stem cell activation and proliferation. Recently, a small peptide highly conserved across the evolution, Apelin, has been shown to improve muscle stem cell proliferation, muscle regeneration and muscular function in aged animals. However, the molecular and cellular mechanisms underlying apelin regenerative function are largely unknown. This project will characterize the effects of apelin as an anti-aging and pro-regenerative signaling peptide and decipher the cell type specific functions of apelin in muscle homeostasis. The Madelaine Lab expects their findings will reveal molecular and cellular therapeutic targets to alleviate or reverse age-associated sarcopenia.
- Breaking Through Fall 2020 Publication · Fall 2020
- Aguillon R, Madelaine R, Aguirrebengoa M, Guturu H, Link S, Dufourcq P, Lecaudey V, Bejerano G, Blader P, Batut J. Morphogenesis is transcriptionally coupled to neurogenesis during peripheral olfactory organ development. Development. 2020 Dec 21;147(24):dev192971. doi: 10.1242/dev.192971. PMID: 33144399.
- Madelaine R, Ngo KJ, Skariah G, Mourrain P. Genetic deciphering of the antagonistic activities of the melanin-concentrating hormone and melanocortin pathways in skin pigmentation. PLoS Genet. 2020 Dec 10;16(12):e1009244. doi: 10.1371/journal.pgen.1009244. PMID: 33301440; PMCID: PMC7755275.
- Leung LC, Wang GX, Madelaine R, Skariah G, Kawakami K, Deisseroth K, Urban AE, Mourrain P. Neural signatures of sleep in zebrafish. Nature. 2019 Jul;571(7764):198-204. doi: 10.1038/s41586-019-1336-7. Epub 2019 Jul 10. PMID: 31292557; PMCID: PMC7081717.
- Postdoctoral Fellow, Stanford University
- Ph.D., Toulouse University, 2011
Student projects using the zebrafish model organism are available to investigate the mechanisms of stem cell dependent regeneration.
Undergraduates will learn basic techniques of molecular and cellular biology, including transgenesis, CRISPR/Cas-9 genome editing, in situ hybridization and immunochemistry.
GSBSE and MBMS grad students from the University of Maine are accepted for rotation projects.
International Master and PhD students are also welcome.
Contact us to discuss your research interest if you are considering joining the lab!
Please see below for Graduate Program opportunities: