- B.S., University of Wyoming, 1999
- Ph.D., University of Utah, 2006
- Postdoc, UC Santa Cruz, 2007-2012
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Germ cells are the progenitors of reproductive cells, and the only cells in sexually reproducing animals that have the potential to give rise to all of the cell types of each subsequent generation. In our lab, we want to understand how these totipotent and immortal properties are conferred. Part of the answer comes from special aggregates called “germ granules” that are found just outside the nucleus of germ cells. Recent research has shown that germ granules play a critical role in maintaining the totipotent and immortal properties of the germline. Take away an organism’s germ granules and it will become sterile. In some organisms, introducing germ granules in cells outside of the germline will restore cellular immortality and totipotency, and will reprogram them into germline stem cells.
Germ granules are conserved in their appearance, subcellular localization, and composition from worms to humans. Because of this, we are able to quickly ascertain the mechanisms by which germ granules function by studying them in a small roundworm called C. elegans. This well-established model organism is responsible for much of what we know about developmental genetics in humans. Using fluorescence microscopy, germ granules (called P granules in C. elegans) can be observed in the germ line of transparent worms at all stages of development. The genetic power of C. elegans combined with its short 3-day generation time and transparency offers an opportunity to study the function of germ granules that isn’t feasible in more complex model organisms.
A focus of our research is to understand the biophysical properties of germ granules, how they are assembled, and the components that affect their subcellular distribution. We have shown that germ granules are held together by weak hydrophobic interactions, and that they extend the size exclusion barrier of the nuclear pore complex out into the cytoplasm of germ cells. Here germ granules create a specialized microenvironment that facilitates post-transcriptional processing events that are exclusive to the germline. We are continuing to investigate the specifics of those processing events to understand how they are used to maintain the totipotent and immortal properties of germ cells, and how these mechanisms can be applied to unlocking stem cell-like properties in other cell types.
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Press Release · June 28, 2018
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Widespread roles for piRNAs and WAGO-class siRNAs in shaping the germline transcriptome of Caenorhabditis elegans. Reed, K.J., Svendsen, J.M., Brown, K.C., Montgomery, B.E., Marks, T.N., Vijayasarathy, T., Parker, D.M., Osborne-Nishimura, E., Updike, D.L., Montgomery, T.A., Nucleic Acids Research. Dec 24, 2019.
Quelling germ cell pluripotency on the genital ridge. Updike, D.L., PNAS Dec 2019, 116(51)25374-5.
Germline maintenance through the multifaceted activities of GLH/Vasa in Caenorhabditis elegans. Marnik, E.A., Fuqua, J.H., Sharp, C.S., Rochester, J.D., Xu, E.L., Holbrook, S.E., & Updike, D.L. Genetics. Nov 2019. 213(3):..
Membraneless organelles: P granules in Caenorhabditis elegans. Marnik, E.A., & Updike, D.L. Traffic. 2019 Jun:20(6):373-379.
ELLI-1, a novel germline protein, modulates RNAi activity and P-granules accumulation in Caenorhabditis elegans. Andralojc, K.M., Campbell, A.C., Kelly, A.L., Terrey, M., Tanner, P.C., Gans, I.M., Senter-Zapata, M.J., Khokhar, E.S., & Updike, D.L. PLoS Genetics. 2017 13(2):e1006611.
PQN-75 is expressed in the pharyngeal gland cells of Caenorhabditis elegans and is dispensable for germline development. Rochester, J.D., Tanner, P.C., Sharp, C.S., Andralojc, K.M., & Updike, D.L. Biology Open 2017 6(9):1355-1363.
Comparative biology of tissue repair, regeneration and aging. Coffman, J.A., Rieger, S., Rogers, A.N., Updike, D.L., & Yin, V.P. npj Regenerative Medicine. 2016 (1).
A forward genetic screen for suppressors of somatic P granules in C. elegans. Kelly, A. L., Senter-Zapata, M. J., Campbell, A. C., Lust, H. E., Theriault, M. E. Andralojc, K. M., & Updike, D.L. G3, 2015 (5),2209-2215.
Specifying and Protecting Germ Cell Fate. Strome S. & Updike D.L. Nature Reviews Molecular Cell Biology. 2015 16(7), 206-216.
CSR-1 and P granules suppress sperm-specific transcription in the C. elegans germline. Campbell AC, Updike DL – Development. 2015 May 15.
Germ-Granule Components Prevent Somatic Development in the C. elegans Germline. Updike DL, Knutson AK, Egelhofer TA, Campbell AC, Strome S – Curr Biol. 2014 Apr 15.
P granules extend the nuclear pore complex environment in the C. elegans Germline. Updike DL, Hachey SJ, Kreher J, Strome S – J Cell Biol. 2012 Mar 21.
P-granule assembly and function in Caenorhabditis elegans germ cells. Updike DL & Strome S – J. Androl. 2010 Jan-Feb.
A genomewide RNAi screen for genes that affect the stability, distribution and function of P granules in C. elegans. Updike DL & Strome S – Genetics 2009 Dec.
Come work with C. elegans and learn genetics! Summer INBRE and NSF REU fellows have used CRISPR/Cas9 technology and both forward and reverse genetics in C. elegans to understand genes that regulate germ-granule biogenesis. Each student is partnered with an experienced technician that will assist them in their project. At the end of the summer, students present their findings in a poster or a talk session at the student symposium.
GSBSE students: We are looking for graduate students to join our lab. Please contact me to discuss potential rotation projects.