Skeletal muscle is a paradigmatic example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare “immortal” stem cells and generating a clonal population of differentiation competent myoblasts. This model currently lacks in vivo validation. Here we define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fibre replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibres and provides in vivo evidence for asymmetric satellite cell division driving both self-renewal and regeneration via a clonally restricted progenitor pool.
Peter Currie, Ph.D. is the Deputy Director of the Australian Regenerative Medicine Institute.
Audience: Scientific Community.
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