Dr. Travis Gould
Associate Professor of Physics
Fluorescence Nanoscopy of Genetic Material
Spatial resolution in fluorescence microscopy is no longer limited by the diffraction of light. By exploiting the photophysical properties of probe molecules, a new class of techniques now provides diffraction-unlimited resolution in fluorescence microscopes. Using either targeted-switching to engineer sub-diffraction focal volumes or the stochastic switching of single fluorophores for precise localization of molecular positions, it has been possible to image biological structures with resolution of a few tens of nanometers. This seminar will review how microscopes became nanoscopes and how stimulated emission depletion (STED) nanoscopy can be applied to image genetic material.