This intensive one-week course in biomedical research protocols and techniques is open to first-year medical students from Yale University School of Medicine. Four biomedical research topics are the focus of this course:
- Physiological studies of chloride transport in an intact epithelial organ;
- Ion channel gene expression in a heterologous expression system;
- Studies in isolated tubule preparations, including immunocytochemistry of phosphorylated vs. non-phosphorylated co-transporters, tissue processing, confocal microscopy, Western blots, and antibody design;
- Cell and molecular biologic methods for study of transporters involved in acid-base disorders and nephrolithiasis: preparation of RNA, cDNA, quantitative PCR, protein electrophoresis and immunoblotting, surface protein biotinylation.
- John N. Forrest Jr., M.D.Professor of Medicine; Director of Student ResearchYale University School of Medicine