Structure and Function of Polarized Epithelial Cells: Yale IPE 2019

This intensive one-week course in biomedical research protocols and techniques is open to first-year medical students from Yale University School of Medicine. Four biomedical research topics are the focus of this course:

1. Physiological studies of chloride transport in an intact epithelial organ;
2. Ion channel gene expression in a heterologous expression system;
3. Studies in isolated tubule preparations, including immunocytochemistry of phosphorylated vs. non-phosphorylated co-transporters, tissue processing, confocal microscopy, Western blots, and antibody design;
4. Cell and molecular biologic methods for study of transporters involved in acid-base disorders and nephrolithiasis: preparation of RNA, cDNA, quantitative PCR, protein electrophoresis and immunoblotting, surface protein biotinylation.