MDI Biological Laboratory

MDI Biological Laboratory
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Quantitative Fluorescence Microscopy 2015

A national, techniques course in fluorescence microscopy and imaging.
(Application deadline: April 15, 2015)
Location: MDI Biological Laboratory (travel information)
Contact Education Office

This one-week, intensive microscopy course will cover all aspects of the technology from the principals of fluorescence imaging to multidimensional imaging in living cells. Lectures dealing with the theory, mechanics, and application of fluorescent imaging methods will be intermingled with extended laboratory practicals in which students will be encouraged to use their own specimens to optimize the utility of the course. Our goal is to provide students with the knowledge and expertise to implement cutting-edge microscopic methods within their own laboratories.

Tuition

$3,100 (includes all course materials, housing, and full meal plan)

Partners

Chroma logoMatTek Corporation logoNikon logo

Course Directors

  • David W. Piston, Ph.D.
    David W. Piston, Ph.D.
    Edward Mallinckrodt, Jr. Professor and Head, Cell Biology and Physiology
    Washington University School of Medicine
  • Simon C. Watkins, Ph.D.
    Simon C. Watkins, Ph.D.
    Distinguished Professor, Cell Biology & Physiology, Founder and Director of the Center for Biologic Imaging
    University of Pittsburgh School of Medicine

Additional Faculty

Anne K. Kenworthy, Ph.D.
Vanderbilt University
Claudette St. Croix, Ph.D.
University of Pittsburgh Graduate School of Public Health
Gert-Jan Kremers, Ph.D.
Imaging Centre Erasmus MC

Friday

16:00 Arrive on campus, check into housing, get keys at Dining Hall

18:00 Dinner and Social at Dining Hall

19:30 Reception at Maren Conference Center and conference material pickup

Saturday

Anytime: Self-serve breakfast in the dining hall

09:00-09:30 Introduction Welcome, Introductions, Define Course Goals (Piston and St. Croix)

09:30-10:30 The Microscope 1 Advances in Modern Imaging for Biological Problems (Kenworthy)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 The Microscope 2 Transmitted Light Contrast in Microscopy (Piston)

12:00-13:00 Lunch

13:00-14:45 LAB 1 TRANSMITTED LIGHT MICROSCOPY

15:00-15:45 The Microscope 3 Fluorescence Microscopy and Quantitation (Piston)

15:45-16:45 Live Cell Imaging 1 Green-Fluorescent Protein (Kremers)

16:45-17:00 Break

17:00-18:00 Digital Imaging 1 Digital Imaging, Resolution, and Detectors (Watkins)

18:00-19:00 Dinner

19:00-20:00 Digital Imaging 2 Optical Filters and Brightness Demonstration (Watkins et al.)

20:30-23:00 LAB 2 STANDARD FLUORESCENCE MICROSCOPY

Sunday

Anytime: Self-serve breakfast in the dining hall

09:00-09:45 Optical Sectioning 1 Introduction to the Confocal Microscope (St Croix)

09:45-10:30 Optical Sectioning 2 Confocal Detectors and “Pseudo-confocals” (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Emerging Methods 1 Super-Resolution (Kenworthy)

12:00-13:00 Lunch

13:00-16:30 LAB 3 OPTICAL SECTIONING MICROSCOPY

16:30-17:30 Tips & Tricks Digital Filters (Watkins)

18:00-19:00 Dinner

19:00-23:00 LAB 4 IMAGE PROCESSING & FEATURE EXTRACTION

Monday

Anytime: Self-serve breakfast in the dining hall

09:00-09:45 Image Processing 1 Practical Digital Imaging (Watkins)

09:45-10:30 Image Processing 2 Considerations for Image Quantitation (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Optical Sectioning 3 3D Methods, Microfluorimetry, Spectral Imaging (St Croix)

12:00 noon Course Photo

12:00-13:00 Lunch

13:00-16:00 LAB 5 MULTICOLOR 3-D IMAGING

16:00-17:30 Tips & Tricks Cell Labeling, Antibodies, Practical Aspects (Watkins)

18:00 Dinner

Tuesday

Anytime: Self-serve breakfast in the dining halll

09:00-10:00 Live Cell Imaging 2 FRAP, Photoactivation, TIRF (Kenworthy)

10:00-10:30 Emerging Methods 2 Light Sheet, SPIM, direct SIM (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Optical Sectioning 4 Deconvolution (Piston)

12:00-13:00 Lunch

13:00-17:00 LAB 6 DECONVOLUTION, SUPER-RESOLUTION

17:00-18:00 Tips & Tricks Live Cell Imaging Design (Watkins)

18:00-19:00 Dinner

19:00-23:00 LAB 7 TIRF, FRAP, PHOTOACTIVATION

Wednesday

Anytime: Self-serve breakfast in the dining hall

09:00-09:45 Live Cell Imaging 3 Multicolor, Ratio Imaging (St Croix)

09:45-10:30 Optical Sectioning 5 Multi-Photon Microscopy (Piston)

10:30-11:00 Break at Maren Conference Center

11:00-12:00 Live Cell Imaging 4 FRET and Live Cell Imaging (Kremers)

12:00-13:00 Lunch

13:00-17:00 LAB 8 LIVE CELL FRET IMAGING

17:00-17:30 LAB 9 SETUP COLLABORATIVE GROUPS FOR LAB 10 BASED ON STUDENT INTERESTS

18:00-19:00 Dinner

19:00-23:00 LAB 10 TIME-LAPSE LIVE CELL IMAGING, TRACKING, QUANTITATION, RATIO IMAGING METHODS, STUDENT EXPERIMENTS

Thursday

Anytime: Self-serve breakfast in the dining hall

09:00-10:00 Image Processing 4 Advanced Image Processing (Watkins)

10:00-10:30 Break at Maren Conference Center

10:30-14:00 LAB 11 ADVANCED IMAGE PROCESSING: 3D QUANTIFICATION, PARTICLE TRACKING; DATA COLLECTION AND PRESENTATION

12:00-13:00 Lunch

14:00-16:00 Results! Student Presentations

16:00-16:30 Mechanics How do I get money to pay for all this stuff? (Watkins)

16:30-17:00 Summing up Q&A, Student Feedback (Faculty)

17:30 Lobster bake

Friday

Move out of housing units no later than 09:00.

On-Campus Housing

Included in tuition. Housing is assigned. Please call 207-288-9880 ext 102 if you have any questions. Subject to availability.

Cottage-style accommodations are available with water front views. Our Cottages sleep 4-5 and are complete with full bathroom, kitchens, living room, high speed wireless internet, and parking.

Dormitory style cottage accommodations are available with double occupancy and single rooms, shared bathrooms, kitchenettes, common room, high speed wireless internet, and parking.

Our on-campus housing is within walking distance of all campus facilities (Note: campus grounds include wooded terrain).

Please visit our Housing Website to view our accommodations. Campus Map

Pond Cottage 14

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