Biomedical Innovation Course

Applications of Organoid Technology

For advanced graduate students, post-doctoral trainees, and researchers at all levels.

May 26–June 1, 2019

Location: MDI Biological Laboratory (travel information)

Overview
Faculty
Schedule
Housing

2019 Organoid Flyer

Applications of Organoid Technology is a one-week intensive course offered by MDI Biological Laboratory in partnership with Hubrecht Organoid Technology (The HUB), a non-profit organization based in the Netherlands. The HUB is founded on the pioneering work of Prof. Dr. Hans Clevers who discovered methods to grow stem cell-derived human epithelial ‘mini-organs’ (organoids) from tissues of patients with various diseases including cancer and cystic fibrosis

Tuition

$3,500.00 USD includes course materials, on-campus double-occupancy lodging, meals, parking, wi-fi.

Partners

Course Directors

Hugo de Jonge, Ph.D.

Professor

Erasmus MC
Robert Vries, Ph.D.

Managing Director

Foundation Hubrecht Organoid Technology

Faculty

Sylvia F. Boj, Ph.D.

Scientific Director

Foundation Hubrecht Organoid Technology
Gert-Jan Kremers, Ph.D.

Director, Imaging Center

Erasmus MC
Leif Oxburgh, Ph.D.

Maine Medical Center Research Institute

Applications of Organoid Technology, 2020: Schedule

Sunday, May 24:

2:00 – 6:00 pm – Arrival of Students

6:00 – 7:00 pm – Dinner

7:00 – 9:00 pm – Introduction to the course (Dr. Forrest, Dr. de Jonge and Dr. Boj)

Monday, May 25:

9:00 – 10:00 am – Lecture: Applications of Organoid Technology (Dr. Boj)

10:00 am – 12:00 pm – Group 1/2: Passaging Organoids (STEM150 + Dog colon) Group 3/4: Plating FIS assay (HUB10)

12:00 – 1:00 pm – Lunch

1:00 – 3:30 pm – Group 1/2: Plating FIS assay (HUB10) Group 3/4: Passaging Organoids (STEM150 + Dog colon)

3:30 – 6:00 pm – Group 1/2: Thawing Organoids (Dog Colon/Colon WT) Group 3/4: Processing for freezing (compact/budding) (B2-47, dog colon)

6:00 – 7:00 pm – Dinner

7:00 – 8:00 pm – Lecture: CF-derived Organoids for Drug Development and Screening (Dr. Boj)

Tuesday, May 26:

8:00 – 10:00 am – Discussion (all groups)

10:00 am – 12:00 pm – Group 1/2: Platting Cell Viability Assay (HUB40) Group 3/4: Running FIS assay (HUB10)

12:00 – 1:00 pm – Lunch

1:00 – 3:30 pm – Group 1/2: Running FIS assay (HUB10) Group 3/4: Platting Cell Viability Assay (HUB40)

3:30 – 6:00 pm – Group 1/2: Processing for freezing (cystic) (STEM150, B2-47) Group 3/4: Thawing Organoids (dog/Colon WT)

6:00 – 7:00 pm – Dinner

7:00 – 8:00 pm – Lecture: 3D Fluorescence Imaging of Organoids (Dr. Kremers)

Wednesday, May 27:

8:00 – 10:00 am – Discussion (all groups)

10:00 am – 12:00 pm – Analysis FIS assay (all groups)

12:00 – 1:00 pm – Lunch

1:00 – 6:00 pm – Available for IPS-derived kidney organoids

6:00 – 7:00 pm – Dinner

7:00 – 8:00 pm – Discussion: How to build an Organoid Biobank and Technical aspects for Culturing Human Organoids (HUB) (HUBj)

Thursday, May 28:

8:00 – 10:00 am – Discussion (all groups)

10:00 – 11:00 am – Refreshing Organoids

11:00 am – 12:00 pm – Lecture: Cancer-derived Organoids for Drug Development and Screening (Dr. Boj)

12:00 – 1:00 pm – Lunch

1:00 – 3:30 pm – Group 1/2 Chamber Assay (HUB10, STEM150) Group 3/4: Freezing Organoids

3:30 – 6:00 pm – Group 1/2 Freezing Organoids Group 3/4: Chamber Assay (HUB10, STEM150)

6:00 – 7:00 pm – Dinner

7:00 – 8:00 pm – Lecture: Available spot for kidney organoids

Friday, May 29:

8:00 – 10:00 am – Discussion (all groups)

10:00 – 11:00 am – Lecture: Cancer-derived Organoids for Drug Development and Screening (Dr. Boj)

11:00 am – 12:30 pm – Measure Cell Viability

12:20 – 1:30 pm – Lunch

1:30 – 6:00 pm – Social Event

6:00 – 8:00 pm – Special Dinner

Saturday: May 30:

8:00 – 10:00 am – Discussion, Survey & Conclusions

10:00 am – Departure of Students

On-campus housing is assigned double occupancy. Single occupancy, if available, can be reserved with an additional fee.