Biomedical Innovation Course
Applications of Organoid Technology
For advanced graduate students, post-doctoral trainees, and researchers at all levels.
Location: MDI Biological Laboratory (travel information)
Applications of Organoid Technology is a one-week intensive course offered by MDI Biological Laboratory in partnership with Hubrecht Organoid Technology (The HUB), a non-profit organization based in the Netherlands. The HUB is founded on the pioneering work of Prof. Dr. Hans Clevers who discovered methods to grow stem cell-derived human epithelial ‘mini-organs’ (organoids) from tissues of patients with various diseases including cancer and cystic fibrosis
Tuition
$3,500.00 USD includes course materials, on-campus double-occupancy lodging, meals, parking, wi-fi.
Partners
Course Directors
Hugo de Jonge, Ph.D.ProfessorErasmus MC
Robert Vries, Ph.D.Managing DirectorFoundation Hubrecht Organoid Technology
Faculty
Sylvia F. Boj, Ph.D.Scientific DirectorFoundation Hubrecht Organoid Technology
Gert-Jan Kremers, Ph.D.Director, Imaging CenterErasmus MC
Leif Oxburgh, Ph.D.Rogosin Institute
Applications of Organoid Technology, 2020: Schedule
Sunday, May 24:
2:00 – 6:00 pm – Arrival of Students
6:00 – 7:00 pm – Dinner
7:00 – 9:00 pm – Introduction to the course (Dr. Forrest, Dr. de Jonge and Dr. Boj)
Monday, May 25:
9:00 – 10:00 am – Lecture: Applications of Organoid Technology (Dr. Boj)
10:00 am – 12:00 pm – Group 1/2: Passaging Organoids (STEM150 + Dog colon) Group 3/4: Plating FIS assay (HUB10)
12:00 – 1:00 pm – Lunch
1:00 – 3:30 pm – Group 1/2: Plating FIS assay (HUB10) Group 3/4: Passaging Organoids (STEM150 + Dog colon)
3:30 – 6:00 pm – Group 1/2: Thawing Organoids (Dog Colon/Colon WT) Group 3/4: Processing for freezing (compact/budding) (B2-47, dog colon)
6:00 – 7:00 pm – Dinner
7:00 – 8:00 pm – Lecture: CF-derived Organoids for Drug Development and Screening (Dr. Boj)
Tuesday, May 26:
8:00 – 10:00 am – Discussion (all groups)
10:00 am – 12:00 pm – Group 1/2: Platting Cell Viability Assay (HUB40) Group 3/4: Running FIS assay (HUB10)
12:00 – 1:00 pm – Lunch
1:00 – 3:30 pm – Group 1/2: Running FIS assay (HUB10) Group 3/4: Platting Cell Viability Assay (HUB40)
3:30 – 6:00 pm – Group 1/2: Processing for freezing (cystic) (STEM150, B2-47) Group 3/4: Thawing Organoids (dog/Colon WT)
6:00 – 7:00 pm – Dinner
7:00 – 8:00 pm – Lecture: 3D Fluorescence Imaging of Organoids (Dr. Kremers)
Wednesday, May 27:
8:00 – 10:00 am – Discussion (all groups)
10:00 am – 12:00 pm – Analysis FIS assay (all groups)
12:00 – 1:00 pm – Lunch
1:00 – 6:00 pm – Available for IPS-derived kidney organoids
6:00 – 7:00 pm – Dinner
7:00 – 8:00 pm – Discussion: How to build an Organoid Biobank and Technical aspects for Culturing Human Organoids (HUB) (HUBj)
Thursday, May 28:
8:00 – 10:00 am – Discussion (all groups)
10:00 – 11:00 am – Refreshing Organoids
11:00 am – 12:00 pm – Lecture: Cancer-derived Organoids for Drug Development and Screening (Dr. Boj)
12:00 – 1:00 pm – Lunch
1:00 – 3:30 pm – Group 1/2 Chamber Assay (HUB10, STEM150) Group 3/4: Freezing Organoids
3:30 – 6:00 pm – Group 1/2 Freezing Organoids Group 3/4: Chamber Assay (HUB10, STEM150)
6:00 – 7:00 pm – Dinner
7:00 – 8:00 pm – Lecture: Available spot for kidney organoids
Friday, May 29:
8:00 – 10:00 am – Discussion (all groups)
10:00 – 11:00 am – Lecture: Cancer-derived Organoids for Drug Development and Screening (Dr. Boj)
11:00 am – 12:30 pm – Measure Cell Viability
12:20 – 1:30 pm – Lunch
1:30 – 6:00 pm – Social Event
6:00 – 8:00 pm – Special Dinner
Saturday: May 30:
8:00 – 10:00 am – Discussion, Survey & Conclusions
10:00 am – Departure of Students
On-campus housing is assigned double occupancy. Single occupancy, if available, can be reserved with an additional fee.