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Molecular Biology Research Techniques: SMCC 2014

A one-week laboratory short course in molecular biology research techniques for undergraduate students from Southern Maine Community College
Location: MDI Biological Laboratory (travel information)
Contact Education Office

This is a hands-on laboratory course in molecular biology for students at Southern Maine Community College . The curriculum will mix intensive, hands-on laboratory work with several informal seminars.

Partner

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  • EEhrenfeld_web
    Elizabeth Ehrenfeld, Ph.D.
    Adjunct Professor of Science
    Southern Maine Community College

Additional Faculty

Greg Mayer, Ph.D.
Texas Tech University
Brian Tarbox, Ph.D.
Southern Maine Community College
Charles Wray, Ph.D.
MDI Biological Laboratory
  Sunday 1/5 Monday 1/6 Tuesday 1/7 Wednesday 1/8 Thursday 1/9 Friday 1/10
AM Lab orientation
DNA extractions
Track A: Fundulus
Track B: Barcoding




Noon: Lunch		 Run gels for Round 1 PCR

Discussion: Basics of Population genetics

Round 2 PCR both tracks
 
 
Noon: Lunch		 Track A: Large scale PCR (Fundulus microsats)

Track B: PCR or Sequence analysis of Barcodes
 
Noon: Lunch		 Gels from PCR Wed.
 
Set up multiplex of all successful PCR and submit to sequencer
 

11 am Alternative Careers Presentation and Q & A Session (Library) with UMPI and UMFK students
Noon: Lunch		 Final data Analysis
 
Using msTools and FStat
 
 
Noon: Lunch
PM DNA quantification

Round 1 PCR		 Two types of
agarose gels for AM PCR


Exo-Sap introduction

Late PM: Track A: How to Submit Microsatellites
Track B: Set up PCR reactions for sequencing		 DNA Sequence analysis introduction

Additional PCR and Sequencing as necessary

Paternity analysis using microsats		 Afternoon:
 
Microbiome analysis: tools techniques and preliminary analysis of Lobster Shell disease (Greg Mayer 1- 3pm)  
Introduction at 1 pm in Library		 Debrief session
 
 
 
 
 
 
Departure by
3 pm
Dinner 6:00pm
7:00 pm Seminar: Course Intro
 		 Dinner at 6:00pm
Reading: Fundulus Pop gen in New England, Duvernell		 Dinner at 6:00pm
Multiplexing PCR for Population Genetics		 Dinner at 6:00pm
Reading: Use of Bar Coding to ID species TBD		 Dinner at 6:00pm
Evening off if possible
 		  

 

Students are encouraged to carpool to the MDI Biological Laboratory. On-campus parking is available adjacent to the dormitories.

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