This is a hands-on laboratory course in developmental biology for students at College of the Atlantic . The curriculum will mix intensive, hands-on laboratory work with several informal seminars.
Students will learn how to use a microscope to observe embryonic development (what to look for) in order to know what normal development looks like. Then they will learn to recognize environmentally-induced abnormal development, and to do so well enough to come up with an informed hypothesis for what aspect of development is perturbed based on what is covered in lecture .
Students will also learn how to measure gene expression using quantitative RT-PCR in order to test hypotheses.
Students will learn how genetic inheritance controls development and how the environment perturbs development by way of altered gene expression.
|Monday 3/24||Tuesday 3/25||Wednesday 3/26||Thursday 3/27||Friday 3/28|
Complete pre-course survey
Course orientation and Lecture 1: Questions and concepts in developmental biology
Training Lab: Fertilization; set up cortisol treatments
Lecture 3: Methods in developmental biology
Training Lab: Examine 1 day embryos under microscope; collect and freeze 1 day samples for RNA prep
|Cancelled due to blizzard(Jim will freeze 2 day embryo samples for qRT-PCR analysis)||Lab: Extract RNA and prepare cDNA from 1d and 2d embryo samplesExamine and image 3 day embryos||Lab: Examine and image 4 day embryos
Q-PCR data analysis
Lecture 2: Developmental plasticity and programming; overview of zebrafish development
Training Lab: Observe early development with stereo microscopes
Lecture 4: Bioinformatics
Training Lab: Microscopic imaging of embryos
|Run Quantitative PCR||Finish Q-PCR data analysisDiscuss plan for remainder of course (weekly Wed afternoon lectures/discussions at COA)
Complete course evaluation
Students reside off campus.